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您好,我购买了贵公司的rm-gsf 和IL-4,用来诱导分化人DC细胞,以及IL-2用以培养人CD8T淋巴细胞,我想问一下推荐的培养液中细胞因子浓度为多少,谢谢!
提问者: 匿名用户 发布时间:2012-02-09 16:13
老师,您好,首先非常感谢您对PeproTech的信任与支持,您试验中所需要添加细胞因子的浓度可以参考以下几篇文献:
1.Cerboni C, Ardolino M, Santoni A,et al.Detuning CD8+ T lymphocytes by down-regulation of the activating receptor NKG2D: role of NKG2D ligands released by activated T cells.Blood. 2009 Mar 26;113(13):2955-64.
2.Saïdi H, Melki MT, Gougeon ML.HMGB1-dependent triggering of HIV-1 replication and persistence in dendritic cells as a consequence of NK-DC cross-talk.PLoS One. 2008;3(10):e3601.
3.Hamzeh-Cognasse H, Cognasse F, Palle S, et al. Direct contact of platelets and their released products exert different effects on human dendritic cell maturation.BMC Immunol. 2008 Sep 25;9:54.
4.Snow AL, Oliveira JB, Zheng L,et al. Critical role for BIM in T cell receptor restimulation-induced death. Biol Direct. 2008 Aug 20;3:34.
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